Population genetic and biophysical evidences reveal that purifying selection shapes the genetic landscape of Plasmodium falciparum RH ligands in Chhattisgarh and West Bengal, India. Non-synonymous amino acid alterations in PfEBA-175 modulate the merozoite ligand's ability to interact with host's Glycophorin A receptor. Group 1 metabotropic glutamate receptors trigger glutamate-induced intracellular Ca2+ signals and nitric oxide release in human brain microvascular endothelial cells. Effects of chemotherapy on placental development and function using in vitro culture of human primary cytotrophoblasts. PGRMC1 phosphorylation affects cell shape, motility, glycolysis, mitochondrial form and function, and tumor growth. Storage Buffer Approximately 0.10.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25☌), 2 SDS. Sizing of proteins on SDS-PAGE and Western blots. Publishing research using ab116028? Please let us know so that we can cite the reference in this datasheet.Īb116028 has been referenced in 16 publications. The BLUelf Prestained Protein Ladder is a three-color protein standard with 13 prestained proteins covering a wide range molecular weights from 3.5 to 245 kilodalton. Now, some additional information: I run mini-gels all the time and never have problems. Storage: Stable for up to 3 months at 4☌. Ladder but no protein - posted in SDS-PAGE and Western Blotting: Hi, I encountered something strange the other day with my western blot: after transfer, the pre-stained ladder (bio-rad) showed up fine, but there was no protein whatsoever on the blot stained with Ponceau. This product was previously called Prism Ultra Protein Ladder (10-245 kDa). Review other protein ladders in the unstained and prestained protein ladder guide.
#PROTEIN LADDER WESTERN BLOT VERIFICATION#
This prestained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE(Tris-glycine buffer).
Prestained Protein Ladder ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa. The BLUeye Prestained Protein Ladder is a three-color protein standard with 12 prestained proteins covering a wide range molecular weights from 10 to 245 kilodalton.
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